Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Low concentration of ethanol induce apoptosis in HepG2 cells: role of various signal transduction pathways. | Int. J. Med. Sci. 2006 3 160 International Journal of Medical Sciences ISSN 1449-1907 2006 3 4 160-167 2006 Ivyspring International Publisher. All rights reserved Research Paper Low concentration of ethanol induce apoptosis in HepG2 cells role of various signal transduction pathways Francisco Castaneda and Sigrid Rosin-Steiner Laboratory for Molecular Pathobiochemistry and Clinical Research Max Planck Institute of Molecular Physiology Dortmund Germany Correspondence to Francisco Castaneda MD Laboratory for Molecular Pathobiochemistry and Clinical Research Max Planck Institute of Molecular Physiology Otto-Hahn-Str. 11 44227 Dortmund Germany. Tel. 49-231-9742-6490 Fax. 49-231-133-2699 E-mail Received Accepted Published As we previously demonstrated in human hepatocellular carcinoma HepG2 cells ethanol at low concentration triggers the Fas apoptotic pathway. However its role in other intracellular signaling pathways remains unknown. Therefore the aim of the present study was to evaluate the role of low concentration of ethanol on different intracellular signaling pathways. For this purpose HepG2 cells were treated with 1 mM ethanol for 10 min and the phosphorylation state of protein kinases was determined. In addition the mRNA levels of transcription factors and genes associated with the Fas apoptotic pathway were determined. Our data demonstrated that ethanol-induced phosphorylation of protein kinases modulates both anti-apoptotic and pro-apoptotic mechanisms in HepG2 cells. Pro-apoptosis resulted mainly from the strong inhibition of the G-protein couple receptor signaling pathway. Moreover the signal transduction initiated by ethanol-induced protein kinases phosphorylation lead to increased expression of the transcription factors with subsequent expression of genes associated with the Fas apoptotic pathway Fas receptor Fas ligand FADD and caspase 8 . These results indicate that .
