Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells. | Int. J. Med. Sci. 2007 4 36 Research Paper International Journal of Medical Sciences ISSN 1449-1907 2007 4 1 36-44 Ivyspring International Publisher. All rights reserved Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells Shin-ichi Ishioka Yoshiaki Ezaka Kota Umemura Takuhiro Hayashi Toshiaki Endo Tsuyoshi Saito Department of Obstetrics and Gynecology Sapporo Medical University School of Medicine Sapporo Japan Correspondence to Shin-ichi Ishioka Department of Obstetrics and Gynecology Sapporo Medical University School of Medicine Mi-nami 1-jo Nishi 16-chome Chuou-ku Sapporo Japan. E-mail ishioka@. Tel 011-611-2111 . Fax 011-563-0860 Received Accepted Published Preeclampsia is often accompanied by hypoxia of the placenta and this condition induces apoptosis in trophoblastic cells. The aim of this study was to characterize global changes of apoptosis-related proteins induced by hypoxia in trophoblastic cells so as to clarify the mechanism of hypoxia-induced apoptosis by using the PoweBlot an antibody-based Western array. Human choriocarcinoma cell line JAR was cultured for 24 hours under aerobic and hypoxic conditions. Hypoxia induced apoptosis accompanied by increased expression of Bcl-x Caspase-3 and -9 Hsp70 PTEN and Bag-1. Bad pan-JNK SAPK-1 Bcl-2 Bid and Caspase-8 showed decreased expression. Hypoxia-induced apoptosis was increased with the transfection of a bag-1 antisense oligonucleotide. The bag-1 antisense oligonucleotide affected the expression of Bid Bad Bcl-2 JNK and phosphorylated JNK although expression of PTEN and Bcl-X did not change. Bag-1 may inhibit apoptosis by suppressing the expression of Bid and Bad. It may also enhance apoptosis by inhibiting the expression of Bcl-2 and by modulating phosphorylation of JNK. Both mitochondrial and stress-activated apoptosis pathways played important roles in the hypoxia induced cell death of trophoblastic .
