Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Characterization of erythrovirus B19 genomes isolated in liver tissues from patients with fulminant hepatitis and biliary atresia who underwent liver transplantation. | Int. J. Med. Sci. 2007 4 105 International Journal of Medical Sciences ISSN 1449-1907 2007 4 2 105-109 Ivyspring International Publisher. All rights reserved Research Paper Characterization of erythrovirus B19 genomes isolated in liver tissues from patients with fulminant hepatitis and biliary atresia who underwent liver transplantation Kenji Abe 1 Tetsuya Kiuchi 2 Koichi Tanaka 3 4 Yoshihiro Edamoto 5 Naoto Aiba 6 and Tetsutaro Sata 1 1. Department of Pathology National Institute of Infectious Diseases Tokyo Japan 2. Department of Transplantation Surgery Nagoya University Hospital Aichi Japan 3. Department of Transplantation Surgery Kyoto University Hospital Kyoto Japan 4. Institute of Biomedical Research and Innovation Hyogo Japan 5. Department of Surgery International Medical Center of Japan Tokyo Japan 6. Aiba Clinic Saitama Japan Correspondence to Dr. Kenji Abe Department of Pathology National Institute of Infectious Diseases Toyama 1-23-1 Shinjuku-ku Tokyo 162-8640 JAPAN. tEl 81 3-5285-1111 ext. 2624 FAX 81 3-5285-1189 E-mail kenjiabe@ Received Accepted Published Background Fulminant hepatitis and biliary atresia are serious problems and their causes have not been explained well. We investigated whether or not erythrovirus B19 is a candidate etiologic agent in such liver disease patients who had undergone liver transplantation. Methods Liver tissues from 47 patients consisted of 28 fulminant hepatitis and 19 biliary atresia were examined to detect B19 genes by PCR and further analyzed their genomic characterization. Results B19 DNA was detected by nested PCR in 10 of 28 cases livers in the fulminant hepatitis group and 7 of 19 livers in the biliary atresia group respectively statistically not significant . Importantly among the 8 hepatic B19 DNA-positive patients who had paired samples of liver and serum the serum B19 genome was detectable in only one case. B19 mRNA was identified in all of 10
