Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN. | Int. J. Med. Sci. 2010 7 147 International Journal of Medical Sciences 2010 7 3 147-154 Ivyspring International Publisher. All rights reserved Research Paper NITRIC OXIDE NO CITRULLINE - NO CYCLE ENZYMES GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA HYPOBARIC HYPOXIA AND REPERFUSION IN RAT BRAIN M. SwamyH Mohd Jamsani Mat Salleh K. N .S. Sirajudeen Wan Roslina Wan Yusof and G. Chandran Department of Chemical Pathology School of Medical Sciences Health campus Universiti Sains Malaysia 16150 Kubang Kerian Kelantan Malaysia ISI Corresponding author Dr. Mummedy Swamy Department of Chemical Pathology School of Medical Sciences Univer-siti Sains Malaysia 16150 Kubang Kerian Kelantan Malaysia. E-mail mswamy@ mummedys@. Fax 609-765 3370 Received Accepted Published Abstract Nitric oxide is postulated to be involved in the pathophysiology of neurological disorders due to hypoxia anoxia in brain due to increased release of glutamate and activation of N-methyl-D-aspartate receptors. Reactive oxygen species have been implicated in pathophysiology of many neurological disorders and in brain function. To understand their role in anoxia hypobaric hypoxia and reperfusion reoxygenation the nitric oxide synthase argi-ninosuccinate synthetase argininosuccinate lyase glutamine synthetase and arginase activities along with the concentration of nitrate nitrite thiobarbituric acid reactive substances and total antioxidant status were estimated in cerebral cortex cerebellum and brain stem of rats subjected to anoxia and reperfusion. The results of this study clearly demonstrated the increased production of nitric oxide by increased activity of nitric oxide synthase. The increased activities of argininosuccinate synthetase and argininosuccinate lyase suggest the increased and effective recycling of citrulline to arginine in anoxia making nitric oxide production more effective and contributing to its toxic effects. The decreased .
