Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: "Cold storage of in vitro cultures of wild chestnut and oak. | 287 Ann Sci For 1995 52 287-293 Elsevier INRA Note Cold storage of in vitro cultures of wild cherry chestnut and oak LV Janeiro AM Vieitez A Ballester Instituto de Investigaciones Agrobiológicas de Galicia CSIC Apartado 122 15080 Santiago de Compostela Spain Received 13 March 1994 accepted 4 October 1994 Summary Shoot cultures of chestnut oak and wild cherry have been stored at low temperature 2 C for 3 6 9 and 12 months. Cultures were stored immediately after the last subculture or 10 d later. Survival and morphogenetic parameters have been recorded at the end of each period of storage. Both survival and proliferation capacity of the explants were influenced by the timing of the transfer. When the explants were stored 10 d after the subculture higher percentages of survival and multiplication rates were obtained. The 4 species studied may be maintained at 2 c for up to 1 year without subculturing. chestnut cold storage in vitro conservation oak wild cherry Resume Conservation au froid de pousses in vitro de merisier Chataignier et chẽne. Des pousses en culture in vitro de Chataignier de chêne et de merlsier ont été conservées à basse temperature 2-C pendant 3. 6 9 et 12 pousses furent stockées juste après le dernier repiquage OU 10 j plus tard. Les taux de survie et les caractéristlques morphologiques ont été déterminées à la fin de chaque période de conservation. La survie comme la capacité de proliferation des explants sont influencees par la durée de la phase de transfert tableaux I II fig 1 . Le stockage des explants 10 j après le repiquage s est traduit par de forts taux de survie et de multiplication. Les 4 espèces étudiées peuvent être maintenues a 2 C pendant plus d une année sans repiquage. Chataignier chêne merisier culture in vitro stockage au froid Correspondence and reprints 288 LV Janeiro et al INTRODUCTION Collections of seeds and clonal material are traditional ways of storing genetic resources. Use of cold stored 0-10 C in vitro cultures
