Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Allergen-specific T cell quantity in blood is higher in allergic compared to nonallergic individuals. | Ueno-Yamanouchi et al. Allergy Asthma Clinical Immunology 2011 7 6 http content 7 1 6 ALLERGY ASTHMA CLINICAL IMMUNOLOGY RESEARCH Open Access Allergen-specific T cell quantity in blood is higher in allergic compared to nonallergic individuals 1 1 13 1 2 Aito Ueno-Yamanouchi Faisal M Khan Bazir Serushago Tom Bowen Cathy Lu Joanne Luider and Jan Storek1 Abstract Background Allergen-specific IgE production is a hallmark of allergic asthma rhinitis eczema. Theoretically this could be due to a high number of allergen-specific B cells or allergen-specific T cells helping allergen-specific B cells differentiate into IgE-producing plasma cells. Here we determined whether the number of allergen-specific B cells or T helper Th cells is higher in allergic individuals compared to nonallergic individuals. Methods A total of 52 allergic individuals and 32 nonallergic individuals were studied. The allergen-specific B and Th cells were enumerated by culturing CFSE-loaded blood mononuclear cells for 7-days with allergen cat Timothy or birch and determining the number of proliferating B or Th cells diluting CFSE by flow cytometry. Allergenspecific IgE concentration was determined by fluorescent enzymoimmunoassay FEIA . Results The quantities of proliferating Th cells but not proliferating B cells specific for cat Timothy and birch were significantly higher in cat- Timothy- and birch-allergic individuals compared to nonallergic individuals. The titer of allergen-specific IgE showed significant correlation with allergen-specific Th cells and not with allergen-specific B cells for all 3 allergens. Conclusions A high number of allergen-specific proliferating Th cells but not proliferating B cells may play a role in the pathogenesis of allergic asthma rhinitis eczema. Background Enhanced production of allergen-specific IgE is characteristic for allergic asthma rhinitis or eczema 1 2 . Upon inhalation ingestion or transcutaneous diffusion of the allergen dendritic
