Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: "Micropropagation and rejuvenation Sequoia sempervirens (Lamb) Endl: a review. | Ann Sci For 1993 50 273-295 Elsevier INRA 273 Review article Micropropagation and rejuvenation of Sequoia sempervirens Lamb Endl a review Y Arnaud 1 A Franclet 2 H Tranvan 1 M Jacques 1 Université p et M Curie Paris VI Laboratoire de Physiologie du Développement des Plantes Tour 53 75252 Paris Cedex 05 2 Institut de Recherche Agronomique et Forestiere 13 avenue de Champabon 77520 Donnemarie-Dontilly France Received 5 May 1992 accepted 17 December 1992 Summary This article describes the botanical biological and forest-tree characteristics of Sequoia sempervirens the reasons for interest in its in vitro vegetative multiplication the difficulty in achieving this from old and remarkable trees and reviews means of overcoming this limitation. Among such means are the repeated culture of stem fragments on media containing appropriate hormonal combinations the micrografting of buds originating from old trees onto juvenile rootstocks and regeneration of buds from previously rejuvenated material. The value and limitations of these protocols and of morphological physiological and biochemical markers of rejuvenation are discussed. The experimental conditions required for the formation of somatic embryos are described. Increased knowledge of in vitro micropropagation will be essential to enhance the use of clonal selection and offer practical outlets to studies concerning somatic hybridization and somatic embryogenesis. micropropagation rejuvenation Sequoia sempervirens I somatic embryogenesis Résumé Micropropagation et rajeunissement du Sequoia sempervirens Lamb Endl revue. Cet article présente les principales caractéristiques botaniques biologiques et forestieres du Sequoia sempervirens. II analyse 1 intérêt de la multiplication vegetative réalisée in vitro soit par micropropagation sensu stricto figs 1 2 et 3 soit par regeneration figs 6 7 et 9 il discute les raisons de la difficulte à la réaliser à partir d arbres âgés et remarquables ainsi que les moyens de la contourner.
