Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Investigation of infectious agents associated with arthritis by reverse transcription PCR of bacterial rRNA. | Available online http content 5 1 R1 Research article Open Access Investigation of infectious agents associated with arthritis by reverse transcription PCR of bacterial rRNA Charles J Cox1 Karen E Kempsell2 and J S Hill Gaston1 Department of Rheumatology University of Cambridge Cambridge 2GlaxoSmithKline Medicines Research Centre Stevenage UK Corresponding author J S Hill Gaston e-mail jshg2@ Received 18 July 2002 Revisions received 11 September 2002 Accepted 13 September 2002 Published 11 October 2002 Arthritis Res Ther 2003 5 R1-R8 DOI ar602 2003 Cox et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any non-commercial purpose provided this notice is preserved along with the article s original URL. Abstract In reactive and postinfeetious arthritis the joints are generally sterile but the presence of bacterial antigens and nucleic acids has been reported. To investigate whether organisms traffic to affected joints in these conditions we performed reverse transcription PCR using universal primers to amplify any bacterial 16S rRNA sequences present in synovial fluid. Bacterial sequences were detected in most cases even after treatment of the synovial fluid with DNase implying the presence of bacterial RNA and therefore of transcriptionally active bacteria. Analysis of a large number of sequences revealed that as reported in rheumatoid arthritis most were derived from gut and skin commensals. Organisms known to have triggered arthritis in each case were not found by sequencing the products obtained using universal primers but could in some cases be shown to be present by amplifying with species specific primers. This was the case for Yersinia pseudotuberculosis and Chlamydia trachomatis. However in arthritis thought to be related to Campylobacter infection the sequences obtained
