Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: κ Exposure to receptor-activator of NFκB ligand renders preγ osteoclasts resistant to IFN-γ by inducing terminal differentiation. | Available online http content 5 1 R49 Research article Open Access Exposure to receptor-activator of NFkB ligand renders preosteoclasts resistant to IFN-y by inducing terminal differentiation Willis Huang1 Regis J O Keefe2 and Edward M Schwarz2 1The Department of Microbiology and Immunology University of Rochester Medical Center Rochester NY USA 2The Center for Musculoskeletal Research University of Rochester Medical Center Rochester NY USA Corresponding author Edward M Schwarz e-mail edward_schwarz@ Received 13 August 2002 Revisions received 11 October 2002 Accepted 14 October 2002 Published 13 November 2002 Arthritis Res Ther 2003 5 R49-R59 DOI ar612 2003 Huang et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any non-commercial purpose provided this notice is preserved along with the article s original URL. Abstract While it has been established that IFN-y is a strong activator of macrophages and a potent inhibitor of osteoclastogenesis in vitro it is also known that this cytokine is produced in particular settings of inflammatory bone loss such as infection and psoriatic arthritis. Because of the different kinetics between rapid IFN-y macrophage activation 24 hours and the slower receptor-activator of NFkB ligand RANKL osteoclast differentiation 7 days we postulated that IFN-y would have different effects on early-stage and late-stage osteoclast precursors. In cells and primary splenocyte cultures pretreatment with RANKL rendered these cells resistant to maximally anti-osteoclastogenic doses of IFN-y. These cells were also resistant to IFN-Y-induced nitric oxide Keywords cellular differentiation cytokines monocytes macrophages production morphological change and surface upregulation of CD11b and receptor-activator of NFkB suggesting that early exposure of .
