Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: All-trans retinoic acid suppresses interleukin-6 expression in interleukin-1-stimulated synovial fibroblasts by inhibition of ERK1/2 pathway independently of RAR activation. | Available online http content 10 6 R141 Research article All-trans retinoic acid suppresses interleukin-6 expression in interleukin-1-stimulated synovial fibroblasts by inhibition of ERK1 2 pathway independently of RAR activation Mélanie Kirchmeyer Meriem Koufany Sylvie Sebillaud Patrick Netter Jean-Yves Jouzeau and Arnaud Bianchi Open Access Laboratoire de Physiopathologie et Pharmacologie Articulaires LPPA UMR 7561 CNRS-Nancy Université 54505 Vandreuvre-lès-Nancy France Corresponding author Arnaud Bianchi Received 5 Jun 2008 Revisions requested 22 Jul 2008 Revisions received 25 Nov 2008 Accepted 1 0 Dec 2008 Published 10 Dec 2008 Arthritis Research Therapy 2008 10 R141 doi ar2569 This article is online at http content 10 6 R141 2008 Kirchmeyer et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction Interleukin-6 IL-6 is thought to play a pathogenic role in rheumatoid arthritis and synovium is a major source of IL-6 release. We investigated the ability of retinoids to suppress IL-6 expression in IL-1-stimulated synovial fibroblasts with special care to the contribution of retinoic acid receptor RAR and retinoid X receptor RXR subtypes and the implication of the mitogen-activated protein kinase MAPK pathway. Methods RAR-a -p and -Y and RXR-a -p and -Ỵ levels were determined by reverse transcription-quantitative polymerase chain reaction RT-qPCR or Western blot in rat synovial fibroblasts stimulated with 10 ng mL of IL-1p. Stimulated levels of IL-6 were assessed by RT-qPCR or immunoassays in the presence or absence of 1 pM aW-trans retinoic acid ATRA RAR agonist or pM BMS-649 RXR agonist . The contribution of .
