Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: κ Increased AP-1 and NF-κB activation and recruitment with the β combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocytes. | Arthritis Research Therapy Vol 6 No 3 Granet et al. Open Access Research article Increased AP-1 and NF-kB activation and recruitment with the combination of the proinflammatory cytokines IL-1P tumor necrosis factor alpha and IL-17 in rheumatoid synoviocytes Corinne Granet1 Wova Maslinski2 and Pierre Miossec1 1Department of Immunology and Rheumatology INSERM U403 Hôpital E Herriot Lyon France 2Department of Pathophysiology and Immunology Institute of Rheumatology Warsaw Poland Corresponding author Pierre Miossec e-mail miossec@ Received 24 Nov 2003 Revisions requested 15 Dec 2003 Revisions received 27 Jan 2004 Accepted 12 Feb 2004 Published 26 Feb 2004 Arthritis Res Ther 2004 6 R190-R198 DOI ar1159 2004 Granet et al. licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract To determine the contribution of IL-1P tumor necrosis factor alpha TNF-a and IL-17 to AP-1 NF-kB and Egr-1 activation in rheumatoid arthritis the effect of the cytokines used alone or in combination was measured on TF expression in rheumatoid synoviocytes. Effects on mRNA expression were measured by RT-PCR and effects on nuclear translocation were measured by immunocytochemistry. To assess the functional consequences of cytokine induction osteoprotegerin levels were measured in synoviocyte supernatants. IL-1P and TNF-a alone at optimal concentration 100 pg ml induced the nuclear translocation of NF-kB and almost all AP-1 members except JunB and Egr-1 for IL-1P and except Fra-2 and Egr-1 for TNF-a. IL-17 was clearly less potent since no nuclear translocation was observed except for a weak activation of Fra-1 and NF-kB. More importantly when these cytokines were used at low concentrations their combination showed a synergistic effect on almost all the TFs except for Egr-1 with a particular effect on .
