Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Improved cartilage integration and interfacial strength after enzymatic treatment in a cartilage transplantation model. | Available online http content 6 5 R469 Research article Improved cartilage integration and interfacial strength after enzymatic treatment in a cartilage transplantation model Jarno van de Breevaart Bravenboer1 Caroline D In der Maur2 P Koen Bos1 Louw Feenstra2 Jan AN Verhaar1 Harrie Weinans1 and Gerjo JVM van Osch1 2 Erasmus Orthopaedic Research Laboratory Department of Orthopaedics Erasmus University Medical Center Rotterdam The Netherlands 2Department of Otorhinolaryngology Erasmus University Medical Center Rotterdam The Netherlands Corresponding author Gerjo JVM van Osch Received 18 Mar 2004 Revisions requested 4 May 2004 Revisions received 30 May 2004 Accepted 23 Jun 2004 Published 6 Aug 2004 Arthritis Res Ther 2004 6 R469-R476 DOI ar1216 2004 van de Breevaart Bravenboer et al. licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Open Access Abstract The objective of the present study was to investigate whether treatment of articular cartilage with hyaluronidase and collagenase enhances histological and mechanical integration of a cartilage graft into a defect. Discs of 3 mm diameter were taken from 8-mm diameter bovine cartilage explants. Both discs and annulus were either treated for 24 hours with hyaluronidase followed by 24 hours with 10 U ml collagenase or left untreated controls . Discs and annulus were reassembled and implanted subcutaneously in nude mice for 5 weeks. Integration of disc with surrounding cartilage was assessed histologically and tested biomechanically by performing a push-out test. After 5 weeks a significant increase in viable cell counts was seen in wound edges of the enzyme-treated group as compared with controls. Furthermore matrix integration expressed as a percentage of the total interface length that .
