Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Morphological and physiological micropropagation. | 121s Ann. Sci. For. 1989 46 suppl. 121s-123s Forest Tree Physiology E. Dreyer etal. eds. Elsevier INRA Morphological and physiological lesions induced by micropropagation A. Harbour1-2 . Atkinson1 K. Oates1 and . Davies1 1 Institute of Environmental and Biological Sciences University of Lancaster Lancaster LA1 4YQ and 2 Neoplants Ltd. Freckleton Lancashire PR4 1HU . Introduction Weaning of plantlets produced in micropropagation often requires an extended period of growth in a fogging facility. This treatment reduces the risk of damage due to desiccation when plantlets are removed from culture. Desiccation injury is a particular problem because of limited development of cuticle abnormal stomatai behaviour and abnormal xylem development in plants raised by these techniques. While effects of culture conditions are generally well understood much less attention has been directed at any morphological and physiological lesions induced by conditions employed during weaning. Photinia fraseri Red Robin is an evergreen woody shrub prized for its bright red young foliage. This plant can be multiplied successfully in culture and will root effectively in compost shortly after transfer from a rooting medium. Problems were observed initially with plants that had been in a fogging house for 20 d. Older leaves of well-rooted plants became flaccid followed rapidly by browning and death of the whole plant. No pathological symptoms were detected. Preliminary observations suggested that a reduced exposure to fogging treatment resulted in reduced plant loss and therefore experiments were conducted where the effects of different weaning conditions on structure and physiology of leaves were investigated. Materials and Methods On 3 different occasions plants were transferred from culture to a peat-based compost contained in modular trays. These trays were placed in the fogging house for 7 d or 21 d before they were moved to the greenhouse. Plants were a fogged in autumn 1987 b .
