Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:Relaxin's induction of metalloproteinases is associated with the loss of collagen and glycosaminoglycans in synovial joint . | Available online http content 7 1 R1 Research article Relaxin s induction of metalloproteinases is associated with the loss of collagen and glycosaminoglycans in synovial joint fibrocartilaginous explants Tabassum Naqvi Trang T Duong Gihan Hashem Momotoshi Shiga Qin Zhang and Sunil Kapila Open Access Department of Orthodontics and Pediatric Dentistry University of Michigan Ann Arbor Michigan USA Contributed equally Corresponding author Sunil Kapila skapila@ Received 20 Jul 2004 Revisions requested 17 Sep 2004 Revisions received 19 Sep 2004 Accepted 27 Sep 2004 Published 29 Oct 2004 Arthritis Res Ther 2005 7 R1-R11 DOI ar1451 2004 Naqvi et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Diseases of specific fibrocartilaginous joints are especially common in women of reproductive age suggesting that female hormones contribute to their etiopathogenesis. Previously we showed that relaxin dose-dependently induces matrix metalloproteinase MMP expression in isolated joint fibrocartilaginous cells. Here we determined the effects of relaxin with or without p-estradiol on the modulation of MMPs in joint fibrocartilaginous explants and assessed the contribution of these proteinases to the loss of collagen and glycosaminoglycan GAG in this tissue. Fibrocartilaginous discs from temporomandibular joints of female rabbits were cultured in medium alone or in medium containing relaxin ng ml or p-estradiol 20 ng ml or relaxin plus p-estradiol. Additional experiments were done in the presence of the MMP inhibitor GM6001 or its control analog. After 48 hours of culture the medium was assayed for MMPs and the discs were analyzed for collagen and GAG concentrations. Relaxin and p-estradiol
