Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:Autoantibodies specific for apoptotic U1-70K are superior serological markers for mixed connective tissue disease. | Available online http content 7 2 R302 Research article Autoantibodies specific for apoptotic U1-70K are superior serological markers for mixed connective tissue disease Danielle Hof1 Kalok Cheung1 Dirk-Jan RAM de Rooij2 Frank H van den Hoogen3 Ger JM Pruijn1 Walther J van Venrooij1 and Jos MH Raats1 4 Department of Biochemistry Nijmegen Centre for Molecular Life Sciences Radboud University Nijmegen Nijmegen The Netherlands department of Rheumatology Sint Maartenskliniek Nijmegen The Netherlands department of Rheumatology University Medical Center St Radboud Nijmegen The Netherlands 4ModiQuest BV Nijmegen The Netherlands Corresponding author Jos MH Raats Received 25 Aug 2004 Revisions requested 25 Oct 2004 Revisions received 26 Nov 2004 Accepted 6 Dec 2004 Published 11 Jan 2005 Arthritis Res Ther 2005 7 R302-R309 DOI ar1490 2005 Hof et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Modifications occurring on autoantigens during cell death have been proposed to have a role in the initiation of autoimmune diseases. Patients suffering from mixed connective tissue disease MCTD produce autoantibodies directed to U1 small nuclear ribonucleoprotein snRNP and antibodies against a 70 kDa protein component the U1-70K 70K protein are the most prominent. During apoptosis 70K is cleaved by caspase-3 to a 40 kDa product which remains associated with the complex. Autoantibodies preferentially recognizing the apoptotic form of 70K have been described previously and an apoptosis-specific epitope on 70K has been identified. This study shows that 29 of 53 54 MCTD sera preferentially recognize the apoptotic form of 70K over intact 70K. Moreover we show that .
