Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Expression of cytokine mRNA and protein in joints and lymphoid organs during the course of rat antigen-induced arthritis. | Available online http content 7 3 R445 Research article Expression of cytokine mRNA and protein in joints and lymphoid organs during the course of rat antigen-induced arthritis Dirk Pohlers1 Angela Siegling2 Eberhard Buchner3 Carsten B Schmidt-Weber4 Ernesta Palombo-Kinne1 Frank Emmrich5 Rolf Brauer6 and Raimund W Kinne1 Open Access Experimental Rheumatology Unit Friedrich Schiller University Jena Jena Germany 2EUCODIS GmbH Vienna Austria 3Pfizer GmbH Karlsruhe Germany 4Swiss Institute for Asthma and Allergy Research SIAF Davos Switzerland institute of Clinical Immunology and Transfusion Medicine University of Leipzig Leipzig Germany institute of Pathology Friedrich Schiller University Jena Jena Germany Corresponding author Raimund W Kinne Received 4 Nov 2004 Revisions requested 3 Dec 2004 Revisions received 4 Jan 2005 Accepted 11 Jan 2005 Published 17 Feb 2005 Arthritis Research Therapy 2005 7 R445-R457 DOI 86 ar1689 2005 Pohlers et a . licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is cited. Abstract Cytokine expression was assessed during antigen-induced arthritis AIA in synovial membrane SM inguinal lymph node LN and spleen using competitive RT-PCR and sandwich ELISA. In the SM early elevations of IL-1 p and IL-6 mRNA by 6 hours 450- and 200-fold respectively correlated with the joint swelling a 6-fold increase in tumor necrosis factor a TNFa was not significant. Not only IL-2 and IFN-Y which increased 10 000-fold and 200-fold respectively but also IL-5 and IL-10 increased acutely 6 hours - day 1 3-fold and 35-fold respectively in the SM. In general the protein levels in the SM for IL-1 p IL-6 TNFa IFN-Y IL-4 and IL-10 increase from 4-fold to 15-fold matched the course of mRNA .
