Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of kinectin as a novel Behçet's disease autoantigen. | Available online http content 7 5 R1133 Open Access Research article Identification of kinectin as a novel Behẹet s disease autoantigen Yu Lu1 2 Ping Ye1 2 Shun-le Chen2 Eng M Tan1 and Edward KL Chan3 Department of Molecular and Experimental Medicine The Scripps Research Institute La Jolla CA USA 2Department of Rheumatology Shanghai Ren Ji Hospital affiliated to Shanghai Second Medical University Shanghai China 3Department of Oral Biology University of Florida Gainesville FL USA Corresponding authors Yu Lu luyu1 00@ Edward KL Chan echan@ Received 14 May 2005 Revisions requested 15 Jun 2005 Revisions received 23 Jun 2005 Accepted 4 Jul 2005 Published 27 Jul 2005 Arthritis Research Therapy 2005 7 R1133-R1139 DOI ar1798 This article is online at http content 7 5 R1133 2005 Lu et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract There has been some evidence that Behget s disease BD has a significant autoimmune component but the molecular identity of putative autoantigens has not been well characterized. In the initial analysis of the autoantibody profile in 39 Chinese BD patients autoantibodies to cellular proteins were uncovered in 23 as determined by immunoblotting. We have now identified one of the major autoantibody specificities using expression cloning. Serum from a BD patient was used as a probe to immunoscreen a XZAP expression cDNA library. Candidate autoantigen cDNAs were characterized by direct nucleotide sequencing and their expressed products were examined for reactivity to the entire panel of BD sera using immunoprecipitation. Reactivity was also examined with normal control sera and disease control sera from patients with lupus and .
