Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of citrullinated α-enolase as a candidate autoantigen in rheumatoid arthritis. | Available online http content 7 6 R1421 Research article Identification of citrullinated a-enolase as a candidate autoantigen in rheumatoid arthritis Andrew Kinloch Verena Tatzer Robin Wait David Peston Karin Lundberg Phillipe Donatien David Moyes Peter C Taylor and Patrick J Venables Kennedy Institute of Rheumatology Imperial College London Charing Cross Hospital Campus 1 Aspenlea Road London W6 8LH UK Corresponding author Patrick J Venables Received 5 May 2005 Revisions requested 1 Jun 2005 Revisions received 8 Sep 2005 Accepted 29 Sep 2005 Published 19 Oct 2005 Arthritis Research Therapy 2005 7 R1421-R1429 DOI ar1845 This article is online at http content 7 6 R1421 2005 Kinloch et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Antibodies against citrullinated proteins are highly specific for rheumatoid arthritis RA but little is understood about their citrullinated target antigens. We have detected a candidate citrullinated protein by immunoblotting lysates of monocytic and granulocytic HL-60 cells treated with peptidylarginine deiminase. In an initial screen of serum samples from four patients with RA and one control a protein of molecular mass 47 kDa from monocytic HL-60s reacted with sera from the patients but not with the serum from the control. Only the citrullinated form of the protein was recognised. The antigen was identified by tandem mass spectrometry as a-enolase and the positions of nine citrulline residues in the sequence were determined. Serum samples from 52 patients with RA and 40 healthy controls were tested for presence of antibodies against citrullinated and non-citrullinated a-enolase by .
