Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:Degradation of small leucine-rich repeat proteoglycans by matrix metalloprotease-13: identification of a new biglycan cleavage site. | Available online http content 8 1 R26 Open Access Research article Degradation of small leucine-rich repeat proteoglycans by matrix metalloprotease-13 identification of a new biglycan cleavage site Jordi Monfort1 Ginette Tardif1 Pascal Reboul1 Franpois Mineau1 Peter Roughley2 Jean-Pierre Pelletier1 and Johanne Martel-Pelletier1 Osteoarthritis Research Unit University of Montreal Hospital Centre Notre-Dame Hospital 1560 Sherbrooke Street East Montreal Quebec H2L 4M1 Canada 2Genetics Unit Shriner s Hospital for Children 1529 Cedar Avenue Montreal Quebec H3G 1A6 Canada Corresponding author Johanne Martel-Pelletier jm@ Received 4 Aug 2005 Revisions requested 14 Sep 2005 Revisions received 25 Nov 2005 Accepted 28 Nov 2005 Published 3 Jan 2006 Arthritis Research Therapy 2006 8 R26 doi ar1 873 This article is online at http content 8 1 R26 2006 Monfort et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract A major and early feature of cartilage degeneration is proteoglycan breakdown. Matrix metalloprotease MMP -13 plays an important role in cartilage degradation in osteoarthritis OA . This MMP in addition to initiating collagen fibre cleavage acts on several proteoglycans. One of the proteoglycan families termed small leucine-rich proteoglycans SLRPs was found to be involved in collagen fibril formation interaction with some members playing a role in the OA process. We investigated the ability of MMP-13 to cleave members of two classes of SLRPs biglycan and decorin and fibromodulin and lumican. SLRPs were isolated from human normal and OA cartilage using guanidinium chloride 4 mol l extraction. Digestion products were examined using Western blotting.
