Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Identification and characterization of the carboxy-terminal region of Sip-1, a novel autoantigen in Behçet's disease. | Available online http content 8 3 R71 Research article Identification and characterization of the carboxy-terminal region of Sip-1 a novel autoantigen in Behẹet s disease Federica Delunardo1 Fabrizio Conti2 Paola Margutti1 Cristiano Alessandri2 Roberta Priori2 Alessandra Siracusano1 Rachele Riganò1 Elisabetta Profumo1 Guido Valesini2 Maurizio Sorice3 and Elena Ortona1 Open Access 1Dipartimento di Malattie Infettive Parassitarie e Immunomediate Istituto Superiore di Sanità Rome Italy 2Dipartimento di Clinica e Terapia Medica Applicata Cattedra di Reumatologia Università La Sapienza Rome Italy 3Dipartimento di Medicina Sperimentale e Patologia Università La Sapienza Rome Italy Corresponding author Elena Ortona ortona@ Received 24 Nov 2005 Revisions requested 4 Jan 2006 Revisions received 23 Feb 2006 Accepted 17 Mar 2006 Published 12 Apr 2006 Arthritis Research Therapy 2006 8 R71 doi ar1940 This article is online at http content 8 3 R71 2006 Delunardo et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Given the lack of a serological test specific for Behget s disease its diagnosis rests upon clinical criteria. The clinical diagnosis is nevertheless difficult because the disease manifestations vary widely especially at the onset of disease. The aim of this study was to identify molecules specifically recognized by serum autoantibodies in patients with Behget s disease and to evaluate their diagnostic value. We screened a cDNA library from human microvascular endothelial cells with serum IgG from two patients with Behget s disease and isolated a reactive clone specific to the carboxy-terminal subunit of Sip1 Sip1 C-ter . Using ELISA we measured IgG IgM and
