Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Radiation Oncology cung cấp cho các bạn kiến thức về ngành y đề tài: Carbonic anhydrase XII expression is associated with histologic grade of cervical cancer and superior radiotherapy outcome. | Yoo et al. Radiation Oncology 2010 5 101 http content 5 1 101 RADIATION ONCOLOGY RESEARCH Open Access Carbonic anhydrase XII expression is associated with histologic grade of cervical cancer and superior radiotherapy outcome 1 f 1 f 1 1 2 3 1 Chong Woo Yoo Byung-Ho Nam Joo-Young Kim Hye-Jin Shin Hyunsun Lim Sun Lee Su-Kyoung Lee Myong-Cheol Lim 1 Yong-Jung Song1 Abstract Background To investigate whether expression of carbonic anhydrase XII CA12 is associated with histologic grade of the tumors and radiotherapy outcomes of the patients with invasive cervical cancer. Methods CA12 expression was examined by immunohistochemical stains in cervical cancer tissues from 183 radiotherapy patients. Histological grading was classified as well WD moderately MD or poorly differentiated PD . Oligonucleotide microarray experiment was performed using seven cervical cancer samples to examine differentially expressed genes between WD and PD cervical cancers. The association between CA12 and histological grade was analyzed by chi-square test. CA12 and histological grades were analyzed individually and as combined CA12 and histologic grade categories for effects on survival outcome. Results Immunohistochemical expression of CA12 was highly associated with the histologic grade of cervical cancer. Lack of CA12 expression was associated with PD histology with an odds ratio of P . Microarray analysis showed a fourfold reduction in CA12 gene expression in PD tumors. CA12 expression was marginally associated with superior disease-free survival. Application of the new combined categories resulted in further discrimination of the prognosis of patients with moderate and poorly differentiated tumor grade. Conclusions Our study indicates that CA12 may be used as a novel prognostic marker in combination with histologic grade of the tumors. Background CA12 is one of the tumor-associated antigens known to be overexpressed under hypoxic conditions. Overexpression of CA12
