Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Radiation Oncology cung cấp cho các bạn kiến thức về ngành y đề tài: The alkylphospholipid, perifosine, radiosensitizes prostate cancer cells both in vitro and in vivo. | Gao et al. Radiation Oncology 2011 6 39 http content 6 1 39 RADIATION ONCOLOGY RESEARCH Open Access The alkylphospholipid perifosine radiosensitizes prostate cancer cells both in vitro and in vivo Yuanhong Gao1 2 3 4 5 Hiromichi Ishiyama1 6 Mianen Sun1 Kathryn L Brinkman1 Xiaozhen Wang1 2 3 7 Julie Zhu1 2 3 1245 23 23 23 1 Weiyuan Mai Ying Huang Daniel Floryk Michael Ittmann Timothy C Thompson E Brian Butler Bo Xu1 and Bin S Teh1 2 3 Abstract Background Perifosine is a membrane-targeted alkylphospholipid developed to inhibit the PI3K Akt pathway and has been suggested as a favorable candidate for combined use with radiotherapy. In this study we investigated the effect of the combined treatment of perifosine and radiation CTPR on prostate cancer cells in vitro and on prostate cancer xenografts in vivo. Methods Human prostate cancer cell line CWR22RV1 was treated with perifosine radiation or CTPR. Clonogenic survival assays sulforhodamine B cytotoxity assays and cell density assays were used to assess the effectiveness of each therapy in vitro. Measurements of apoptosis cell cycle analysis by flow cytometry and Western blots were used to evaluate mechanisms of action in vitro. Tumor growth delay assays were used to evaluate radiation induced tumor responses in vivo. Results In vitro CTPR had greater inhibitory effects on prostate cancer cell viability and clonogenic survival than either perifosine or radiation treatment alone. A marked increase in prostate cancer cell apoptosis was noted in CTPR. Phosphorylation of AKT-T308 AKT and S473 were decreased when using perifosine treatment or CTPR. Cleaved caspase 3 was significantly increased in the CTPR group. In vivo CTPR had greater inhibitory effects on the growth of xenografts when compared with perifosine or radiation treatment alone groups. Conclusions Perifosine enhances prostate cancer radiosensitivity in vitro and in vivo. These data provide strong support for further development of this
