Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Radiation Oncology cung cấp cho các bạn kiến thức về ngành y đề tài: Increased betulinic acid induced cytotoxicity and radiosensitivity in glioma cells under hypoxic conditions. | Bache et al. Radiation Oncology 2011 6 111 http content 6 1 111 RADIATION ONCOLOGY RESEARCH Open Access Increased betulinic acid induced cytotoxicity and radiosensitivity in glioma cells under hypoxic conditions 1 1 Ỷ I- rx 1 Ỷ 1 1 Matthias Bache Martin P Zschornak Sarina Passin Jacqueline KeBler Henri Wichmann Matthias Kappler D I kx -X r I D-x r- -kx l z-x3 M 1Z-X 11 I z xr xr z I r 3 I_I -X r I r kx IZ Z X m m V -X 3 I_I I z t I -X I I L-xx-xir-t-2 4 -X V X Z-J I r f Ị f zz 1 zx ZV V X -X fl 1 Reinhard Paschke Goran N Kaiuoerovic Harish Kommera Helge lauoert ano Dirk voroermark Abstract Background Betulinic acid BA is a novel antineoplastic agent under evaluation for tumor therapy. Because of the selective cytotoxic effects of BA in tumor cells including gliomas the combination of this agent with conservative therapies such as radiotherapy and chemotherapy may be useful. Previously the combination of BA with irradiation under hypoxic conditions had never been studied. Methods In this study the effects of 3 to 30 pM BA on cytotoxicity migration the protein expression of PARP survivin and HIF-1a as well as radiosensitivity under normoxic and hypoxic conditions were analyzed in the human malignant glioma cell lines U251MG and U343MG. Cytotoxicity and radiosensitivity were analyzed with clonogenic survival assays migration was analyzed with Boyden chamber assays or scratch assays and protein expression was examined with Western blot analyses. Results Under normoxic conditions a half maximal inhibitory concentration IC50 of 23 pM was observed in U251MG cells and 24 pM was observed in U343MG cells. Under hypoxic conditions 10 pM or 15 pM of BA showed a significantly increased cytotoxicity in U251MG cells p and p respectively and U343MG cells p and p respectively . The combination of BA with radiotherapy resulted in an additive effect in the U343MG cell line under normoxic and hypoxic conditions. Weak radiation enhancement .
