Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Hypoxic conditions increase hypoxia-inducible transcription factor 2α and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients. | Available online http content 9 3 R55 Research article Hypoxic conditions increase hypoxia-inducible transcription factor 2a and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients Wasim S Khan Adetola B Adesida and Timothy E Hardingham UK Centre for Tissue Engineering and Wellcome Trust Centre for Cell Matrix Research Faculty of Life Sciences Michael Smith Building University of Manchester Oxford Road Manchester M13 9PT UK Corresponding author Timothy E Hardingham Received 1 Feb 2007 Revisions requested 21 Mar 2007 Revisions received 11 Mar 2007 Accepted 30 May 2007 Published 30 May 2007 Arthritis Research Therapy 2007 9 R55 doi ar2211 This article is online at http content 9 3 R55 2007 Khan et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Stem cells derived from the infrapatellar fat pad IPFP are a potential source of stem cells for the repair of articular cartilage defects. Hypoxia has been shown to improve chondrogenesis in adult stem cells. In this study we investigated the effects of hypoxia on gene expression changes and chondrogenesis in stem cells from the IPFP removed from elderly patients with osteoarthritis at total knee replacement. Adherent colonyforming cells were isolated and cultured from the IPFP from total knee replacement. The cells at passage 2 were characterised for stem cell surface epitopes and then cultured for 14 days as cell aggregates in chondrogenic medium under normoxic 20 oxygen or hypoxic 5 oxygen conditions. Gene expression analysis DNA and glycosoaminoglycan assays and immunohistochemical staining were determined to assess chondrogenesis.
