Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Glucosamine prevents in vitro collagen degradation in chondrocytes by inhibiting advanced lipoxidation reactions and protein oxidation. | Available online http content 9 4 R76 Research article Glucosamine prevents in vitro collagen degradation in chondrocytes by inhibiting advanced lipoxidation reactions and protein oxidation Moti L Tiku Haritha Narla Mohit Jain and Praveen Yalamanchili Open Access Department of Medicine University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School One Robert Wood Johnson Place New Brunswick NJ 08903 USA Corresponding author Moti L Tiku tikuml@ Received 2 Nov 2006 Revisions requested 10 Jan 2007 Revisions received 5 Jul 2007 Accepted 8 Aug 2007 Published 8 Aug 2007 Arthritis Research Therapy 2007 9 R76 doi ar2274 This article is online at http content 9 4 R76 2007 Tiku et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Osteoarthritis OA affects a large segment of the aging population and is a major cause of pain and disability. At present there is no specific treatment available to prevent or retard the cartilage destruction that occurs in OA. Recently glucosamine sulfate has received attention as a putative agent that may retard cartilage degradation in OA. The precise mechanism of action of glucosamine is not known. We investigated the effect of glucosamine in an in vitro model of cartilage collagen degradation in which collagen degradation induced by activated chondrocytes is mediated by lipid peroxidation reaction. Lipid peroxidation in chondrocytes was measured by conjugated diene formation. Protein oxidation and aldehydic adduct formation were studied by immunoblot assays. Antioxidant effect of glucosamine was also tested on malondialdehyde thiobarbituric acid-reactive substances TBARS formation on purified lipoprotein .
