Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Cyclooxygenase inhibition lowers prostaglandin E2 release from articular cartilage and reduces apoptosis but not proteoglycan degradation following an impact load in vitro. | Available online http content 9 6 R1 29 Research article Cyclooxygenase inhibition lowers prostaglandin E2 release from articular cartilage and reduces apoptosis but not proteoglycan degradation following an impact load in vitro Janet E Jeffrey and Richard M Aspden Department of Orthopaedic Surgery University of Aberdeen Institute of Medical Sciences Foresterhill Aberdeen AB25 2ZD UK Corresponding author Janet E Jeffrey Received 8 Feb 2007 Revisions requested 21 Mar 2007 Revisions received 14 Oct 2007 Accepted 20 Dec 2007 Published 20 Dec 2007 Arthritis Research Therapy 2007 9 R129 doi ar2346 This article is online at http content 9 6 R1 29 2007 Jeffrey and Aspden licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract This study investigated the release of prostaglandin E2 PGE2 from cartilage following an impact load in vitro and the possible chondroprotective effect of cyclooxygenase-2 COX-2 inhibition using non-steroidal anti-inflammatory drugs NSAIDs . Explants of human articular cartilage were subjected to a single impact load in a drop tower and then cultured for 6 days in the presence of either a selective COX-2 inhibitor celecoxib and 10 pM or a non-selective COX inhibitor indomethacin and 10 pM . The concentrations of PGE2 and glycosaminoglycans GAGs a measure of cartilage breakdown were measured in the explant culture medium at 3 and 6 days post-impact. Apoptotic cell death was measured in frozen explant sections by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling TUNEL method. PGE2 levels were increased by more than 20-fold in the medium of explants at both 3 p and 6 days p .
