Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Regulation of Sox9 activity by crosstalk with nuclear factor-κB and retinoic acid receptors. | Available online http content 10 1 R3 Research article Regulation of Sox9 activity by crosstalk with nuclear factor-KB and retinoic acid receptors Jason S Rockel Julie C Kudirka Andrew J Guzi and Suzanne M Bernier Open Access Canadian Institutes of Health Research Group in Skeletal Development and Remodeling and Department of Anatomy and Cell Biology Schulich School of Medicine Dentistry The University of Western Ontario London Ontario Canada N6A 5C1 Corresponding author Jason S Rockel Received 13 Sep 2007 Revisions requested 19 Oct 2007 Revisions received 8 Nov 2007 Accepted 9 Jan 2008 Published 9 Jan 2008 Arthritis Research Therapy 2008 10 R3 doi 1 ar2349 This article is online at http content 10 1 R3 2008 Rockel et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction Sox9 and p300 cooperate to induce expression of cartilage-specific matrix proteins including type II collagen aggrecan and link protein. Tumour necrosis factor TNF -a found in arthritic joints activates nuclear factor-KB NF-kB whereas retinoic acid receptors RARs are activated by retinoid agonists including all-trans retinoic acid atRA . Like Sox9 the activity of NF-kB and RARs depends upon their association with p300. Separately both TNF-a and atRA suppress cartilage matrix gene expression. We investigated how TNF-a and atRA alter the expression of cartilage matrix genes. Methods Primary cultures of rat chondrocytes were treated with TNF-a and or atRA for 24 hours. Levels of transcripts encoding cartilage matrix proteins were determined by Northern blot analyses and quantitative real-time PCR. Nuclear protein levels DNA binding and functional activity of .
