Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Human articular chondrocytes produce IL-7 and respond to IL-7 with increased production of matrix metalloproteinase-13. | Available online http content 10 1 R23 Research article Human articular chondrocytes produce IL-7 and respond to IL-7 with increased production of matrix metalloproteinase-13 David Long1 Simon Blake2 Xiao-Yu Song2 Michael Lark2 and Richard F Loeser1 Section of Molecular Medicine Department of Internal Medicine Wake Forest University School of Medicine Medical Center Blvd Winston-Salem North Carolina 27157 USA 2Centocor Inc. Great Valley Parkway Malvern Pennsylvania 19355 USA Corresponding author Richard F Loeser rloeser@ Received 29 Jun 2007 Revisions requested 29 Aug 2007 Revisions received 29 Jan 2008 Accepted 20 Feb 2008 Published 20 Feb 2008 Arthritis Research Therapy 2008 10 R23 doi ar2376 This article is online at http content 10 1 R23 2008 Long et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Fibronectin fragments have been found in the articular cartilage and synovial fluid of patients with osteoarthritis and rheumatoid arthritis. These matrix fragments can stimulate production of multiple mediators of matrix destruction including various cytokines and metalloproteinases. The purpose of this study was to discover novel mediators of cartilage destruction using fibronectin fragments as a stimulus. Methods Human articular cartilage was obtained from tissue donors and from osteoarthritic cartilage removed at the time of knee replacement surgery. Enzymatically isolated chondrocytes in serum-free cultures were stimulated overnight with the 110 kDa a5p1 integrin-binding fibronectin fragment or with IL-1 IL-6 or IL-7. Cytokines and matrix metalloproteinases released into the media were detected using antibody arrays and .
