Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Dynamic compression counteracts IL-1β induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs. | Available online http content 10 2 R35 Open Access Research article Dynamic compression counteracts IL-1 p induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte agarose constructs TT Chowdhury1 S Arghandawi1 J Brand2 OO Akanji1 DL Bader1 DM Salter2 and DA Lee1 School of Engineering and Materials Science Queen Mary University of London Mile End Road London E1 4NS UK 2Queens Medical Research Institute 47 Little France Cresent Edinburgh University EH16 4TJ. UK Corresponding author TT Chowdhury Received 27 Sep 2007 Revisions requested 27 Nov 2007 Revisions received 28 Feb 2008 Accepted 18 Mar 2008 Published 18 Mar 2008 Arthritis Research Therapy 2008 10 R35 doi ar2389 This article is online at http content 10 2 R35 2008 Chowdhury et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Nitric oxide and prostaglandin E2 PGE2play pivotal roles in both the pathogenesis of osteoarthritis and catabolic processes in articular cartilage. These mediators are influenced by both IL-1p and mechanical loading and involve alterations in the inducible nitric oxide synthase iNOS and cyclo-oxygenase COX -2 enzymes. To identify the specific interactions that are activated by both types of stimuli we examined the effects of dynamic compression on levels of expression of iNOS and COX-2 and involvement of the p38 mitogen-activated protein kinase MAPK pathway. Methods Chondrocyte agarose constructs were cultured under free-swelling conditions with or without IL-1p and or SB203580 inhibitor of p38 MAPK for up to 48 hours. Using a fully characterized bioreactor system constructs were subjected to dynamic compression for 6
