Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Expression of novel extracellular sulfatases Sulf-1 and Sulf-2 in normal and osteoarthritic articular cartilage. | Available online http content 10 3 R61 Research article Expression of novel extracellular sulfatases Sulf-1 and Sulf-2 in normal and osteoarthritic articular cartilage Shuhei Otsuki1 Noboru Taniguchi1 Shawn P Grogan1 Darryl D Lima1 Mitsuo Kinoshita2 and Martin Lotz1 Division of Arthritis Research The Scripps Research Institute 10550 North Torrey Pines Road La Jolla CA 92037 USA 2Department of Orthopedic Surgery Osaka Medical College 2-7 Daigaku-machi Takatsuki 569-8686 Osaka Japan Corresponding author Shuhei Otsuki otsuki@ Received 14 Jan 2008 Revisions requested 18 Feb 2008 Revisions received 4 Apr 2008 Accepted 28 May 2008 Published 28 May 2008 Arthritis Research Therapy 2008 10 R61 doi ar2432 This article is online at http content 10 3 R61 2008 Otsuki et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Changes in sulfation of cartilage glycosaminoglycans as mediated by sulfatases can regulate growth factor signaling. The aim of this study was to analyze expression patterns of recently identified extracellular sulfatases Sulf-1 and Sulf-2 in articular cartilage and chondrocytes. Methods Sulf-1 and Sulf-2 expressions in human articular cartilage from normal donors and patients with osteoarthritis OA and in normal and aged mouse joints were analyzed by real-time polymerase chain reaction immunohistochemistry and Western blotting. Results In normal articular cartilage Sulf-1 and Sulf-2 mRNAs and proteins were expressed predominantly in the superficial zone. OA cartilage showed significantly higher Sulf-1 and Sulf- 2 mRNA expression as compared with normal human articular cartilage. Sulf protein expression in OA cartilage was
