Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells. | Available online http content 10 3 R65 Research article Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells Francois NK Kwong1 Stephen M Richardson2 and Christopher H Evans1 Center for Molecular Orthopaedics Harvard Medical School Longwood Avenue Boston Massachusetts 02115 USA 2Tissue Injury and Repair Group The University of Manchester Oxford Road Manchester M13 9PT UK Corresponding author Francois NK Kwong fcng@ Received 20 Dec 2007 Revisions requested 7 Feb 2008 Revisions received 11 May 2008 Accepted 4 Jun 2008 Published 4 Jun 2008 Arthritis Research Therapy 2008 10 R65 doi ar2436 This article is online at http content 10 3 R65 2008 Kwong et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Bone morphogenetic proteins BMPs are critical growth factors in the osteogenic differentiation of progenitor cells during development in embryos and fracture repair in adults. Although recombinant BMPs are in use clinically their clinical efficiency needs to be improved. The biological activities of BMPs are naturally regulated by extracellular binding proteins. The specific hypotheses tested in this study were as follows the BMP inhibitor chordin is produced endogenously during the osteogenic differentiation of human mesenchymal stem cells MSCs and blockade of the activity of the BMP inhibitor increases the rate of osteogenic differentiation of human MSCs in vitro. Methods Human MSCs were derived from bone marrow from an iliac crest aspirate and from patients undergoing hip hemiarthroplasty. The MSCs were induced down the osteogenic pathway using standard osteogenic differentiation media and .
