Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix. | Available online http content 10 4 R89 Research article Adipose-derived mesenchymal stem cells from the sand rat transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix Hazel Tapp Ray Deepe Jane A Ingram Marshall Kuremsky Edward N Hanley Jr and Helen E Gruber Department of Orthopaedic Surgery 1000 Blythe Blvd Carolinas Medical Center Charlotte NC 28232 USA Corresponding author Hazel Tapp Received 23 Apr 2008 Revisions requested 5 Jun 2008 Revisions received 18 Jun 2008 Accepted 11 Aug 2008 Published 11 Aug 2008 Arthritis Research Therapy 2008 10 R89 doi ar2473 This article is online at http content 10 4 R89 2008 Tapp et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Adult mesenchymal stem cell therapy has a potential application in the biological treatment of disc degeneration. Our objectives were to direct adipose-derived mesenchymal stem cells AD-MSC from the sand rat to produce a proteoglycan and collagen type I extracellular matrix ECM rich in known ECM components of the annulus fibrosis of disc and to stimulate proteoglycan production by co-culture of human annulus cells with AD-MSC. Methods AD-MSC were isolated and characterised by adherence to plastic appropriate expression of cluster of differentiation CD markers and differentiation to osteoblasts and chondrocytes in vitro. AD-MSC were grown in threedimensional 3D culture and treated with or without transforming growth factor beta TGFp to direct them to produce annulus-like ECM as determined by proteoglycan content and collagen expression. AD-MSC were .
