Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: 4-Hydroxynonenal induces apoptosis in human osteoarthritic chondrocytes: the protective role of glutathione-S-transferase. | Available online http content 10 5 R107 Research article 4-Hydroxynonenal induces apoptosis in human osteoarthritic chondrocytes the protective role of glutathione-S-transferase France Vaillancourt Hassan Fahmi Qin Shi Patrick Lavigne Pierre Ranger Julio C Fernandes and Mohamed Benderdour Orthopaedic Research Laboratory Hôpital du Sacré-C ur de Montreal Department of Surgery University of Montreal 5400 Gouin Blvd. West Montreal QC H4J 1C5 Canada Corresponding author Mohamed Benderdour Received 23 Jun 2008 Revisions requested 15 Jul 2008 Revisions received 16 Aug 2008 Accepted 9 Sep 2008 Published 9 Sep 2008 Arthritis Research Therapy 2008 10 R107 doi ar2503 This article is online at http content 10 5 R107 2008 Vaillancourt et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction 4-Hydroxynonenal HNE is one of the most abundant and reactive aldehydes of lipid peroxidation products and exerts various effects on intracellular and extracellular signalling cascades. We have previously shown that HNE at low concentrations could be considered as an important mediator of catabolic and inflammatory processes in osteoarthritis OA . In the present study we focused on characterizing the signalling cascade induced by high HNE concentration involved in cell death in human OA chondrocytes. Methods Markers of apoptosis were quantified with commercial kits. Protein levels were evaluated by Western blotting. Glutathione GSH and ATP levels were measured with commercial kits. Glucose uptake was assessed by 2-deoxy-D- 3H -glucose. The role of GSH-S-transferase A4-4 GSTa4-4 in controlling HNE-induced chondrocyte apoptosis was .
