Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: The effects of infliximab therapy on the serum proteome of rheumatoid arthritis patients. | Available online http content 11 2 R32 Research article The effects of infliximab therapy on the serum proteome of rheumatoid arthritis patients Ravi C Dwivedi1 2 Navjot Dhindsa2 Oleg V Krokhin1 2 John Cortens1 2 John A Wilkins1 2 and Hani S El-Gabalawy1 2 Manitoba Centre for Proteomics and Systems Biology University of Manitoba 799-715 McDermot Avenue Winnipeg MB R3E 3P4 Canada 2Department of Internal Medicine University of Manitoba RR1 49-800 Sherbrook Street Winnipeg MB R3A 1M4 Canada Corresponding author Hani S El-Gabalawy elgabalh@ Received 7 Jan 2009 Revisions requested 30 Jan 2009 Revisions received 25 Feb 2009 Accepted 6 Mar 2009 Published 6 Mar 2009 Arthritis Research Therapy 2009 11 R32 doi ar2637 This article is online at http content 11 2 R32 2009 Dwivedi et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Although the clinical effects of infliximab therapy in rheumatoid arthritis have been documented extensively the biological effects of this intervention continue to be defined. We sought to examine the impact of infliximab therapy on the serum proteome of rheumatoid arthritis patients by means of a mass spectrometry-based approach. Methods Sera from 10 patients with rheumatoid arthritis were obtained prior to and following 12 weeks of infliximab therapy using a standard clinical protocol. The sera were immunodepleted of the 12 highest abundance proteins labeled by the iTRAQ isobaric tagging for relative and absolute protein quantification technique and analyzed by mass spectrometry to identify proteomic changes associated with treatment. Results An average of 373 distinct proteins were identified per .
