Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Candidate autoantigens identified by mass spectrometry in early rheumatoid arthritis are chaperones and citrullinated glycolytic enzymes. | Available online http content 11 2 R38 Research article Candidate autoantigens identified by mass spectrometry in early rheumatoid arthritis are chaperones and citrullinated glycolytic enzymes Vincent Goeb1 Marlène Thomas-L Otellier2 Romain Daveau2 Roland Charlionet2 Patrice Fardellone3 Xavier Le Loet1 Franọois Tron2 Danièle Gilbert2 and Olivier Vittecoq1 Department of Rheumatology and Inserm Unit 905 IFRMP 23 Institute for Biomedical Research University of Rouen Rouen University Hospital Rouen 76031 cedex France 2Immunology Laboratory and Inserm Unit 905 IFRMP 23 Institute for Biomedical Research University of Rouen Rouen University Hospital Rouen 76031 cedex France 3Rheumatology Department Amiens University Hospital Amiens 80054 France Corresponding author Vincent Goeb goebvince@ Received 10 Jun 2008 Revisions requested 15 Jul 2008 Revisions received 26 Jan 2009 Accepted 10 Mar 2009 Published 10 Mar 2009 Arthritis Research Therapy 2009 11 R38 doi ar2644 This article is online at http content 11 2 R38 2009 Goeb et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction The aim of our study was to identify new early rheumatoid arthritis RA autoantibodies. Methods Sera obtained from 110 early untreated RA patients 6 months were analyzed by western blot using HL-60 cell extract separated on one-dimensional and two-dimensional gel electrophoresis 1-DE 2-DE . Sera from 50 healthy blood donors and 20 patients with non-RA rheumatisms were used as controls for 1-DE and 2-DE respectively. The immunoreactive proteins were identified by MALDI-TOF mass spectrometric analysis and the presence of potential sites of citrullination in each of these
