Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Mesenchymal progenitor cell markers in human articular cartilage: normal distribution and changes in osteoarthritis. | Available online http content 11 3 R85 Research article Mesenchymal progenitor cell markers in human articular cartilage normal distribution and changes in osteoarthritis Shawn P Grogan1 2 Shigeru Miyaki1 Hiroshi Asahara1 Darryl D D Lima1 2 and Martin K Lotz1 Department of Molecular and Experimental Medicine The Scripps Research Institute 10550 North Torrey Pines Road La Jolla California 92037 USA 2Shiley Center for Orthopaedic Research and Education at Scripps Clinic 11025 North Torrey Pines Road Suite 1 40 La Jolla California 92037 USA Corresponding author Martin K Lotz mlotz@ Received 24 Feb 2009 Revisions requested 1 Apr 2009 Revisions received 7 May 2009 Accepted 5 Jun 2009 Published 5 Jun 2009 Arthritis Research Therapy 2009 11 R85 doi ar2719 This article is online at http content 11 3 R85 2009 Grogan et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Recent findings suggest that articular cartilage contains mesenchymal progenitor cells. The aim of this study was to examine the distribution of stem cell markers Notch-1 Stro-1 and VCAM-1 and of molecules that modulate progenitor differentiation Notch-1 and Sox9 in normal adult human articular cartilage and in osteoarthritis OA cartilage. Methods Expression of the markers was analyzed by immunohistochemistry IHC and flow cytometry. Hoechst 33342 dye was used to identify and sort the cartilage side population SP . Multilineage differentiation assays including chondrogenesis osteogenesis and adipogenesis were performed on SP and non-SP NSP cells. Results A surprisingly high number 45 of cells were positive for Notch-1 Stro-1 and VCAM-1 throughout normal cartilage. .
