Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Short- and long-term effects of anti-CD20 treatment on B cell ontogeny in bone marrow of patients with rheumatoid arthritis. | Available online http content 11 4 R123 Research article Short- and long-term effects of anti-CD20 treatment on B cell ontogeny in bone marrow of patients with rheumatoid arthritis Maria Rehnberg Sylvie Amu Andrej Tarkowski Maria I Bokarewa and Mikael Brisslert Open Access Department of Rheumatology and Inflammation Research Sahlgrenska Academy at University of Gothenburg Guldhedsgatan 10A 413 46 Gothenburg Sweden Corresponding author Mikael Brisslert Received 10 Mar 2009 Revisions requested 20 Apr 2009 Revisions received 29 Jul 2009 Accepted 17 Aug 2009 Published 17 Aug 2009 Arthritis Research Therapy 2009 11 R123 doi 86 ar2789 This article is online at http content 11 4 R123 2009 Rehnberg et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction In the present study we evaluated changes in the B cell phenotype in peripheral blood and bone marrow BM of patients with rheumatoid arthritis RA following anti-CD20 treatment using rituximab. Methods Blood and BM samples were obtained from 37 patients with RA prior to rituximab treatment. Ten of these patients were resampled 1 month following rituximab 14 patients after 3 months and the remaining 13 patients were included in the long-term follow up. B cell populations were characterized by CD27 IgD CD38 CD24 expression. Results One and three months following rituximab BM retained up to 30 of B cells while circulation was totally depleted of B cells. Analysis of the remaining BM B cells showed prevalence of immature and or transitional B cells CD38 CD24 and CD27 IgD- memory cells while IgD cells were completely depleted. A significant reduction of CD27 cells in BM and in .
