Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Downregulation of heat shock protein 70 protects rheumatoid arthritis fibroblast-like synoviocytes from nitric oxide-induced apoptosis. | Available online http content 11 4 R130 Research article Downregulation of heat shock protein 70 protects rheumatoid arthritis fibroblast-like synoviocytes from nitric oxide-induced apoptosis Eun Ha Kang1 Dong Jo Kim2 Eun Young Lee1 Yun Jong Lee1 Eun Bong Lee1 and Yeong Wook Song1 Division of Rheumatology Department of Internal Medicine Rheumatism Research Institute Seoul National University Hospital 28 Yongun-dong Chongno-gu Seoul 110-744 Republic of Korea 2Department of Biochemistry Seoul National University College of Medicine 28 Yongun-dong Chongno-gu Seoul 110-799 Republic of Korea Corresponding author Yeong Wook Song ysong@ Received 22 Apr 2009 Revisions requested 13 Jul 2009 Revisions received 2 Aug 2009 Accepted 27 Aug 2009 Published 27 Aug 2009 Arthritis Research Therapy 2009 11 R130 doi 86 ar2797 This article is online at http content 11 4 R130 2009 Kang et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Heat shock protein 70 Hsp70 is a well-known anti-apoptotic protein that blocks multiple steps in the stress-induced apoptotic pathway. Enhanced Hsp70 expression has previously been demonstrated in rheumatoid arthritis RA fibroblast-like synoviocytes FLSs . The authors investigated the role of Hsp70 in the survival of RA FLSs in a sodium nitroprusside SNP -treated environment. Methods Targeted knock-down of Hsp70 was performed by RNA interference in RA FLSs at passage 3-7. After SNP treatment the morphological features of apoptosis were observed by phase-contrast microscopy. Cell survival was measured by MTT 3- 4 5-dimethylthiazol-2-yl -2 5-diphenyltetrazolium bromide assays and by flow cytometric analysis after .
