Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. | Zhang et al. Respiratory Research 2010 11 39 http content 11 1 39 RESPIRATORY RESEARCH RESEARCH Open Access Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats Shui-Juan Zhang21 2 Yang-Mei Deng21 Yi-Liang Zhu 1 Xin-Wei Dong1 Jun-Xia Jiang1 and Qiang-Min Xie 1 Abstract Background Basic and clinical studies suggest that hypothalamic-pituitary-adrenal HPA axis is the neuroendocrine-immnue pathway that functionally regulates the chronic inflammatory disease including asthma. Our previous studies showed corresponding changes of cytokines and leukotriene B4 LTB4 between brain and lung tissues in antigen-challenged asthmatic rats. Here we investigated how the increased LTB4 level in brain interacts with HPA axis in regulating antigen-induced asthmatic response in sensitized rats. Methods Ovalbumin-sensitized rats were challenged by inhalation of antigen. Rats received vehicle LTB4 or U75302 a selective LTB4 BLT1 receptor inhibitor was given via intracerebroventricular injection 30 min before challenge. Lung resistance RL and dynamic lung compliance Cdyn were measured before and after antigen challenge. Inflammatory response in lung tissue was assessed 24 h after challenge. Expression of CRH mRNA and protein in hypothalamus were evaluated by RT-PCR and Western Blot and plasma levels of adrenocorticotropic hormone ACTH and corticosterone CORT were measured using the ELISA kits. Results Antigen challenge decreased pulmonary function and induced airway inflammation evoked HPA axis response in sensitized rats. Administration of LTB4 via markedly attenuated airway contraction and inflammation. Meanwhile LTB4 via markedly increased CORT and ACTH level in plasma before antigen challenge and followed by further increases in CORT and ACTH levels in plasma after antigen challenge in sensitized rats. Expression of CRH mRNA and protein in .
