Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Deregulation of apoptosis mediators' p53 and bcl2 in lung tissue of COPD patients. | Siganaki et al. Respiratory Research 2010 11 46 http content 11 1 46 RESPIRATORY RESEARCH RESEARCH Open Access Deregulation of apoptosis mediators p53 and bcl2 in lung tissue of COPD patients Marianna Siganaki 1 Anastasios V Koutsopoulos42 Eirini Neofytou41 Eleni Vlachaki3 Maria Psarrou1 Nikolaos Soulitzis1 4 Nikolaos Pentilas5 Sophia Schiza3 Nikolaos M Siafakas1 3 and Eleni G Tzortzaki 1 3 Abstract Abnormal apoptotic events in chronic obstructive pulmonary disease COPD subvert cellular homeostasis and may play a primary role in its pathogenesis. However studies in human subjects are limited. p53 and bcl2 protein expression was measured by western blot on lung tissue specimens from 43 subjects 23 COPD smokers and 20 non-COPD smokers using beta-actin as internal control. Additionally p53 and bcl2 expression patterns were evaluated by immunohistochemistry in formalin-fixed paraffin-embedded lung tissue sections from the same individuals. Western blot analysis showed statistically significant increased p53 protein levels in COPD smokers in comparison with non-COPD smokers p while bcl2 protein levels were not statistically different between the two groups. Lung immunohistochemistry showed increased ratio of positive p53-stained type II pneumocytes total type II pneumocytes in COPD smokers compared to non-COPD smokers p whereas the p53 staining ratio in alveolar macrophages and in lymphocyte-like cells did not differ statistically between the two groups. On the other hand bcl2 expression did not differ between the two groups in all three cell types. The increased expression of pro-apoptotic p53 in type II pneumocytes of COPD patients not counterbalanced by the anti-apoptotic bcl2 could reflect increased apoptosis in the alveolar epithelium of COPD patients. Our results confirm previous experiments and support the hypothesis of a disturbance in the balance between the pro- and anti-apoptotic mediators in COPD. Introduction COPD is a .
