Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Respiratory allergy to Blomia tropicalis: Immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model. | Baqueiro et al. Respiratory Research 2010 11 51 http content 11 1 51 RESPIRATORY RESEARCH RESEARCH Open Access Respiratory allergy to Blomia tropicalis Immune response in four syngeneic mouse strains and assessment of a low allergen-dose short-term experimental model Tiana Baqueiro 1 2 Momtchilo Russo3 Virginia MG Silva1 4 Thayna Meirelles4 Pablo RS Oliveira4 Eliane Gomes3 Renato Barboza3 Ana T Cerqueira-Lima1 Camila A Figueiredo1 Lain Pontes-de-Carvalho4 and Neuza M Alcântara-Neves 1 Abstract Background The dust mite Blomia tropicalis is an important source of aeroallergens in tropical areas. Although a mouse model for B. tropicalisextract BtE -induced asthma has been described no study comparing different mouse strains in this asthma model has been reported. The relevance and reproducibility of experimental animal models of allergy depends on the genetic background of the animal the molecular composition of the allergen and the experimental protocol. Objectives This work had two objectives. The first was to study the anti-B. tropicalis allergic responses in different mouse strains using a short-term model of respiratory allergy to BtE. This study included the comparison of the allergic responses elicited by BtE with those elicited by ovalbumin in mice of the strain that responded better to BtE sensitization. The second objective was to investigate whether the best responder mouse strain could be used in an experimental model of allergy employing relatively low BtE doses. Methods Groups of mice of four different syngeneic strains were sensitized subcutaneously with 100 pg of BtE on days 0 and 7 and challenged four times intranasally at days 8 10 12 and 14 with 10 pg of BtE. A J mice that were the best responders to BtE sensitization were used to compare the B. tropicalis-specific asthma experimental model with the conventional experimental model of ovalbumin OVA -specific asthma. A J mice were also sensitized with a lower dose of BtE. .
