Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Mucosal sensitization to German cockroach involves protease-activated receptor-2. | Page et al. Respiratory Research 2010 11 62 http content 11 1 62 RESPIRATORY RESEARCH RESEARCH Open Access Mucosal sensitization to German cockroach involves protease-activated receptor-2 Kristen Page 1 2 John R Ledford1 Ping Zhou1 Krista Dienger1 and Marsha Wills-Karp1 2 Abstract Background Allergic asthma is on the rise in developed countries. A common characteristic of allergens is that they contain intrinsic protease activity and many have been shown to activate protease-activated receptor PAR -2 in vitro. The role for PAR-2 in mediating allergic airway inflammation has not been assessed using a real world allergen. Methods Mice wild type or PAR-2-deficient were sensitized to German cockroach GC feces frass or protease-depleted GC frass by either mucosal exposure or intraperitoneal injection and measurements of airway inflammation IL-5 IL-13 IL-17A and IFNy levels in the lung serum IgE levels cellular infiltration mucin production and airway hyperresponsiveness were performed. Results Following systemic sensitization GC frass increased airway hyperresponsiveness Th2 cytokine release serum IgE levels cellular infiltration and mucin production in wild type mice. Interestingly PAR-2-deficient mice had similar responses as wild type mice. Since these data were in direct contrast to our finding that mucosal sensitization with GC frass proteases regulated airway hyperresponsiveness and mucin production in BALB c mice Page et. al. 2007 Resp Res 8 91 we backcrossed the PAR-2-deficient mice into the BALB c strain. Sensitization to GC frass could now occur via the more physiologically relevant method of intratracheal inhalation. PAR-2-deficient mice had significantly reduced airway hyperresponsiveness Th2 and Th17 cytokine release serum IgE levels and cellular infiltration compared to wild type mice when sensitization to GC frass occurred through the mucosa. To confirm the importance of mucosal exposure mice were systemically sensitized to GC .
