Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Response of the mouse lung transcriptome to welding fume: effects of stainless and mild steel fumes on lung gene expression in A/J and C57BL/6J mice. | Zeidler-Erdely et al. Respiratory Research 2010 11 70 http content 11 1 70 RESPIRATORY RESEARCH RESEARCH Open Access Response of the mouse lung transcriptome to welding fume effects of stainless and mild steel fumes on lung gene expression in A J and C57BL 6J mice Patti C Zeidler-Erdely 1 Michael L Kashon2 Shengqiao Li2 and James M Antonini1 Abstract Background Debate exists as to whether welding fume is carcinogenic but epidemiological evidence suggests that welders are an at risk population for the development of lung cancer. Recently we found that exposure to welding fume caused an acutely greater and prolonged lung inflammatory response in lung tumor susceptible A J versus resistant C57BL 6J B6 mice and a trend for increased tumor incidence after stainless steel SS fume exposure. Here our objective was to examine potential strain-dependent differences in the regulation and resolution of the lung inflammatory response induced by carcinogenic Cr and Ni abundant or non-carcinogenic iron abundant metalcontaining welding fumes at the transcriptome level. Methods Mice were exposed four times by pharyngeal aspiration to 5 mg kg iron abundant gas metal arc-mild steel GMA-MS Cr and Ni abundant GMA-SS fume or vehicle and were euthanized 4 and 16 weeks after the last exposure. Whole lung microarray using Illumina Mouse Ref-8 expression beadchips was done. Results Overall we found that tumor susceptibility was associated with a more marked transcriptional response to both GMA-MS and -SS welding fumes. Also Ingenuity Pathway Analysis revealed that gene regulation and expression in the top molecular networks differed between the strains at both time points post-exposure. Interestingly a common finding between the strains was that GMA-MS fume exposure altered behavioral gene networks. In contrast GMA-SS fume exposure chronically upregulated chemotactic and immunomodulatory genes such as CCL3 CCL4 CXCL2 and MMP12 in the A J strain. In the .
