Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Obstructive apneas induce early activation of mesenchymal stem cells and enhancement of endothelial wound healing. | Carreras et al. Respiratory Research 2010 11 91 http content 11 1 91 RESPIRATORY RESEARCH RESEARCH Open Access Obstructive apneas induce early activation of mesenchymal stem cells and enhancement of endothelial wound healing Alba Carreras 1 2 Mauricio Rojas3 Theodora Tsapikouni1 2 Josep M Montserrat2 4 Daniel Navajas1 2 5 and Ramon Farre 1 2 Abstract Background The aim was to test the hypothesis that the blood serum of rats subjected to recurrent airway obstructions mimicking obstructive sleep apnea OSA induces early activation of bone marrow-derived mesenchymal stem cells MSC and enhancement of endothelial wound healing. Methods We studied 30 control rats and 30 rats subjected to recurrent obstructive apneas 60 per hour lasting 15 s each for 5 h . The migration induced in MSC by apneic serum was measured by transwell assays. MSC-endothelial adhesion induced by apneic serum was assessed by incubating fluorescent-labelled MSC on monolayers of cultured endothelial cells from rat aorta. A wound healing assay was used to investigate the effect of apneic serum on endothelial repair. Results Apneic serum showed significant increase in chemotaxis in MSC when compared with control serum the normalized chemotaxis indices were m SE and respectively p . MSC adhesion to endothelial cells was greater -fold p in apneic serum than in control serum. When compared with control serum apneic serum significantly increased endothelial wound healing -fold p . Conclusions The early increases induced by recurrent obstructive apneas in MSC migration adhesion and endothelial repair suggest that these mechanisms play a role in the physiological response to the challenges associated to OSA. Background Obstructive sleep apnea OSA is a prevalent disease affecting both children and adults. This sleep breathing disorder caused by an abnormal increase in upper airway collapsibility is characterized by recurrent events
