Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Pneumocystis cell wall β-glucan stimulates calcium-dependent signaling of IL-8 secretion by human airway epithelial cells. | Carmona et al. Respiratory Research 2010 11 95 http content 11 1 95 RESPIRATORY RESEARCH RESEARCH Open Access Pneumocystis cell wall p-glucan stimulates calcium-dependent signaling of IL-8 secretion by human airway epithelial cells Eva M Carmona Jeffrey D Lamont Ailing Xue Mark Wylam and Andrew H Limper Abstract Background Respiratory failure secondary to alveolar inflammation during Pneumocystis pneumonia is a major cause of death in immunocompromised patients. Neutrophil infiltration in the lung of patients with Pneumocystis infection predicts severity of the infection and death. Several previous studies indicate that airway epithelial cells release the neutrophil chemoattractant proteins MIP-2 rodents and IL-8 humans in response to Pneumocystis and purified Pneumocystis cell wall p-glucans PCBG through the NF-KB-dependent pathway. However little is known about the molecular mechanisms that are involved in the activation of airway epithelium cells by PCBG resulting in the secretion of IL-8. Method To address this we have studied the activation of different calcium-dependent mitogen-activated protein kinases MAPKs in 1HAEo- cells a human airway epithelial cell line. Results Our data provide evidence that PCBG induces phosphorylation of the MAPKs ERK and p38 the activation of NF-kB and the subsequently secretion of IL-8 in a calcium-dependent manner. Further we evaluated the role of glycosphingolipids as possible receptors for p-glucans in human airway epithelial cells. Preincubation of the cells with D-tbreo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol PDMP a potent inhibitor of the glycosphingolipids synthesis prior to PCBG stimulation significantly decreased IL-8 production. Conclusion These data indicate that PCBG activates calcium dependent MAPK signaling resulting in the release of IL-8 in a process that requires glycosphingolipid for optimal signaling. Introduction Pneumocystis pneumonia is an opportunistic infection caused by .
