Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Expression and activation of the oxytocin receptor in airway smooth muscle cells: Regulation by TNFa and IL-13. | Amrani et al. Respiratory Research 2010 11 104 http content 11 1 104 RESPIRATORY RESEARCH RESEARCH Open Access Expression and activation of the oxytocin receptor in airway smooth muscle cells Regulation by TNFa and IL-13 A t 1t 1 1 1 2 2 Yassine Amrani Farhat Syed Chris Huang Katherine Li Veronica Liu Deepika Jain Stefan Keslacy 22 2 2 4 4 Michael W Sims Hasna Baidouri Philip R Cooper Hengjiang Zhao Salman Siddiqui Christopher E Brightling Don Griswold1 Lily Li3 Reynold A Panettieri Jr2 Abstract Background During pregnancy asthma may remain stable improve or worsen. The factors underlying the deleterious effect of pregnancy on asthma remain unknown. Oxytocin is a neurohypophyseal protein that regulates a number of central and peripheral responses such as uterine contractions and milk ejection. Additional evidence suggests that oxytocin regulates inflammatory processes in other tissues given the ubiquitous expression of the oxytocin receptor. The purpose of this study was to define the role of oxytocin in modulating human airway smooth muscle HASMCs function in the presence and absence of IL-13 and TNFa cytokines known to be important in asthma. Method Expression of oxytocin receptor in cultured HASMCs was performed by real time PCR and flow cytomery assays. Responses to oxytocin was assessed by fluorimetry to detect calcium signals while isolated tracheal rings and precision cut lung slices PCLS were used to measure contractile responses. Finally ELISA was used to compare oxytocin levels in the bronchoalveloar lavage BAL samples from healthy subjects and those with asthma. Results PCR analysis demonstrates that OXTR is expressed in HASMCs under basal conditions and that both interleukin IL -13 and tumor necrosis factor TNFa stimulate a time-dependent increase in OXTR expression at 6 and 18 hr. Additionally oxytocin increases cytosolic calcium levels in fura-2-loaded HASMCs that were enhanced in cells treated for 24 hr with IL-13. .
