Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Detection of Chlamydia trachomatis-DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems. | Available online http content 11 6 R175 Research article Detection of Chlamydia trachomatis-DNA in synovial fluid evaluation of the sensitivity of different DNA extraction methods and amplification systems Julia Freise1 Iris Bernau2 Sabine Meier3 Henning Zeidler4 and Jens G Kuipers5 Division of Pneumology Hannover Medical School Carl-Neuberg StraBe 1 Hannover 30625 Germany 2Division of Anaesthesiology Diako Hospital Gropelinger HeerstraBe 406 - 408 Bremen 28239 Germany 3Division of Immunology and Rheumatology Hannover Medical School Carl-Neuberg StraBe. 1 Hannover 30625 Germany 4Rheumatologikum Rathenau-StraBe 13-14 Hannover 30159 Germany 5Division of Rheumatology Rotes Kreuz Krankenhaus 24 Bremen 28199 Germany Contributed equally Corresponding author Julia Freise juliafreise@ Received 16 May 2009 Revisions requested 18 Jun 2009 Revisions received 14 Oct 2009 Accepted 21 Nov 2009 Published 21 Nov 2009 Arthritis Research Therapy 2009 11 R175 doi 86 ar2864 This article is online at http content 11 6 R1 75 2009 Freise et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Polymerase chain reaction PCR and ligase chain reaction LCR are used in research for detection of Chlamydia trachomatis C. tr. in synovial fluid SF . However there is no standardized system for diagnostic use in clinical practice therefore this study aimed at determining the molecular biology method best suited to detect C. tr. from SF. Methods SF samples were spiked with C. tr. elementary bodies EB and human peripheral blood monocytes PBMo persistently infected with C. tr. in vitro to evaluate the sensitivity of different molecular biology methods
