Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Dimethylthiourea protects against chlorine induced changes in airway function in a murine model of irritant induced asthma. | McGovern et al. Respiratory Research 2010 11 138 http content 11 1 138 RESPIRATORY RESEARCH RESEARCH Open Access Dimethylthiourea protects against chlorine induced changes in airway function in a murine model of irritant induced asthma Toby K McGovern 1 William S Powell1 Brian J Day2 Carl W White2 Karuthapillai Govindaraju1 Harry Karmouty-Quintana1 Normand Lavoie1 Ju Jing Tan1 James G Martin1 Abstract Background Exposure to chlorine Cl2 causes airway injury characterized by oxidative damage an influx of inflammatory cells and airway hyperresponsiveness. We hypothesized that Cl2-induced airway injury may be attenuated by antioxidant treatment even after the initial injury. Methods Balb C mice were exposed to Cl2 gas 100 ppm for 5 mins an exposure that was established to alter airway function with minimal histological disruption of the epithelium. Twenty-four hours after exposure to Cl2 airway responsiveness to aerosolized methacholine MCh was measured. Bronchoalveolar lavage BAL was performed to determine inflammatory cell profiles total protein and glutathione levels. Dimethylthiourea DMTU 100 mg kg was administered one hour before or one hour following Cl2 exposure. Results Mice exposed to Cl2 had airway hyperresponsiveness to MCh compared to control animals pre-treated and post-treated with DMTU. Total cell counts in BAL fluid were elevated by Cl2 exposure and were not affected by DMTU treatment. However DMTU-treated mice had lower protein levels in the BAL than the Cl2-only treated animals. 4-Hydroxynonenal analysis showed that DMTU given pre- or post-Cl2 prevented lipid peroxidation in the lung. Following Cl2 exposure glutathione GSH was elevated immediately following exposure both in BAL cells and in fluid and this change was prevented by DMTU. GSSG was depleted in Cl2 exposed mice at later time points. However the GSH GSSG ratio remained high in chlorine exposed mice an effect attenuated by DMTU. Conclusion Our data show that the .
