Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Expression and function of human hemokinin-1 in human and guinea pig airways. | Grassin-Delyle et al. Respiratory Research 2010 11 139 http content 11 1 139 RESPIRATORY RESEARCH RESEARCH Open Access Expression and function of human hemokinin-1 in human and guinea pig airways Stanislas Grassin-Delyle1 Emmanuel Naline1 Amparo Buenestado1 Paul-André Risse1 2 Edouard Sage3 Charles Advenier1 Philippe Devillier1 Abstract Background Human hemokinin-1 hHK-1 and endokinins are peptides of the tachykinin family encoded by the TAC4 gene. TAC4 and hHK-1 expression as well as effects of hHK-1 in the lung and airways remain however unknown and were explored in this study. Methods RT-PCR analysis was performed on human bronchi to assess expression of tachykinin and tachykinin receptors genes. Enzyme immunoassay was used to quantify hHK-1 and effects of hHK-1 and endokinins on contraction of human and guinea pig airways were then evaluated as well as the role of hHK-1 on cytokines production by human lung parenchyma or bronchi explants and by lung macrophages. Results In human bronchi expression of the genes that encode for hHK-1 tachykinin NK1-and NK2-receptors was demonstrated. hHK-1 protein was found in supernatants from explants of human bronchi lung parenchyma and lung macrophages. Exogenous hHK-1 caused a contractile response in human bronchi mainly through the activation of NK2-receptors which blockade unmasked a NK1-receptor involvement subject to a rapid desensitization. In the guinea pig trachea hHK-1 caused a concentration-dependant contraction mainly mediated through the activation of NK1-receptors. Endokinin A B exerted similar effects to hHK-1 on both human bronchi and guinea pig trachea whereas endokinins C and D were inactive. hHK-1 had no impact on the production of cytokines by explants of human bronchi or lung parenchyma or by human lung macrophages. Conclusions We demonstrate endogenous expression of TAC4 in human bronchi the encoded peptide hHK-1 being expressed and involved in contraction of human and guinea pig
