Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Decreased Toll-like receptor 8 expression and lower TNF-alpha synthesis in infants with acute RSV infection. | Bendelja et al. Respiratory Research 2010 11 143 http content 11 1 143 RESPIRATORY RESEARCH RESEARCH Open Access Decreased Toll-like receptor 8 expression and lower TNF-alpha synthesis in infants with acute RSV infection 1 1 2 3 3 Kreso Bendelja Valerija Vojvoda Neda Aberle Jasna Cepin-Bogovic Alenka Gagro Gordana Mlinaric-Galinovic4 Sabina Rabatic1 Abstract Background Toll-like receptors TLRs are part of the innate immune system able to recognize pathogen-associated molecular patterns and activate immune system upon pathogen challenge. Respiratory syncytial virus RSV is a RNA virus particularly detrimental in infancy. It could cause severe lower respiratory tract disease and recurrent infections related to inadequate development of anti-viral immunity. The reason could be inadequate multiple TLRs engagement including TLR8 in recognition of single-stranded viral RNA and diminished synthesis of inflammatory mediators due to a lower expression. Methods Intracellular TLR8 expression in peripheral blood monocytes from RSV-infected infants was profiled and compared to healthy adults and age matched controls. Whether the observed difference in TLR8 expression is a transitory effect infants in convalescent phase 4-6 weeks later were retested. Specific TLR8-mediated TNF-a production in monocytes during an acute and convalescent phase was analyzed. Results RSV-infected and healthy infants had lower percentage of TLR8-expressing monocytes than healthy adults whereas decreased of TLR8 protein levels were detected only for RSV-infected infant group. Lower protein levels of TLR8 in monocytes from RSV-infected infants compared to healthy infants negatively correlated with respiratory frequency and resulted in lower TNF-a synthesis upon a specific TLR8 stimulation. In the convalescent phase levels of TLR8 increased accompanied by increased TNF-a synthesis compared to acute infection. Conclusions Lower TLR8 expression observed in monocytes during an acute
